tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine (Brule et al., 2004 [PubMed 15248782]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for TRMT5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TRMT5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TRMT5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:60,648,454–60,651,189 | 330.2 kb | Distal (>10kb) Multiome HiCAR | 790 | |
| chr14:60,655,802–60,657,953 | 323.8 kb | Distal (>10kb) Multiome HiCAR | 631 | |
| chr14:60,720,299–60,724,768 | 256.7 kb | Distal (>10kb) Multiome HiCAR | 933 | |
| chr14:60,980,625–60,981,938 | 114 bp | At TSS Multiome | 866 | |
| chr14:61,254,867–61,255,650 | 274.1 kb | Distal (>10kb) Multiome HiCAR | 219 | |
| chr14:61,280,136–61,281,846 | 300.4 kb | Distal (>10kb) Multiome | 391 |
Genomic view of the TRMT5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.