Transcription factors with Perturb-seq knockdown data for TRG-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TRG-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TRG-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:37,915,973–37,917,388 | 414.2 kb | Distal (>10kb) Multiome HiCAR | 641 | |
| chr7:38,177,739–38,178,868 | 152.9 kb | Distal (>10kb) Multiome | 828 | |
| chr7:38,329,010–38,330,278 | 1.6 kb | Proximal (<10kb) Multiome | 103 | |
| chr7:38,330,757–38,331,521 | 25 bp | At TSS Multiome | 166 | |
| chr7:38,331,896–38,332,124 | 786 bp | At TSS | 106 | |
| chr7:38,332,228–38,332,845 | 1.1 kb | Proximal (<10kb) | 201 | |
| chr7:38,335,451–38,336,337 | 4.7 kb | Proximal (<10kb) Multiome | 492 | |
| chr7:38,367,971–38,368,664 | 37.2 kb | Distal (>10kb) Multiome | 205 | |
| chr7:38,369,681–38,370,425 | 39.0 kb | Distal (>10kb) Multiome | 295 | |
| chr7:38,630,623–38,632,074 | 300.2 kb | Distal (>10kb) Multiome | 373 |
Genomic view of the TRG-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.