This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
Transcription factors with Perturb-seq knockdown data for TOP3A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TOP3A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TOP3A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:16,835,071–16,835,593 | 1479.5 kb | Distal (>10kb) Multiome HiCAR | 72 | |
| chr17:18,035,659–18,036,594 | 278.9 kb | Distal (>10kb) Multiome | 186 | |
| chr17:18,038,656–18,040,279 | 275.7 kb | Distal (>10kb) Multiome | 839 | |
| chr17:18,153,365–18,154,352 | 161.0 kb | Distal (>10kb) Multiome | 244 | |
| chr17:18,157,790–18,158,725 | 156.8 kb | Distal (>10kb) Multiome | 292 | |
| chr17:18,182,585–18,185,065 | 131.5 kb | Distal (>10kb) Multiome | 1051 | |
| chr17:18,224,148–18,226,173 | 90.3 kb | Distal (>10kb) Multiome | 680 | |
| chr17:18,246,777–18,247,912 | 67.7 kb | Distal (>10kb) Multiome | 314 | |
| chr17:18,257,865–18,259,083 | 56.3 kb | Distal (>10kb) Multiome | 684 | |
| chr17:18,260,145–18,261,582 | 54.3 kb | Distal (>10kb) Multiome | 778 | |
| chr17:18,314,513–18,315,715 | 163 bp | At TSS Multiome | 865 | |
| chr17:18,363,090–18,363,830 | 48.6 kb | Distal (>10kb) Multiome | 760 |
Genomic view of the TOP3A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.