The protein encoded by this gene is thought to be a negative regulator of NF-kappa-B mediated transcription. The encoded protein may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the DNA. Phosphorylation of this protein targets it for degradation by the ubiquitination pathway, which frees the NF-kappa-B complexes to enter the nucleus. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for TONSL. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TONSL upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TONSL, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:144,146,820–144,149,073 | 296.5 kb | Distal (>10kb) Multiome | 790 | |
| chr8:144,266,293–144,267,098 | 177.9 kb | Distal (>10kb) Multiome | 162 | |
| chr8:144,290,981–144,292,410 | 152.9 kb | Distal (>10kb) Multiome | 716 | |
| chr8:144,315,833–144,316,450 | 128.3 kb | Distal (>10kb) Multiome | 396 | |
| chr8:144,325,768–144,327,719 | 117.5 kb | Distal (>10kb) Multiome | 1016 | |
| chr8:144,331,281–144,333,847 | 112.4 kb | Distal (>10kb) Multiome | 406 | |
| chr8:144,336,102–144,339,119 | 107.6 kb | Distal (>10kb) Multiome | 922 | |
| chr8:144,353,907–144,355,227 | 89.9 kb | Distal (>10kb) Multiome HiCAR | 280 | |
| chr8:144,358,142–144,359,070 | 85.9 kb | Distal (>10kb) Multiome HiCAR | 719 | |
| chr8:144,369,349–144,369,916 | 74.7 kb | Distal (>10kb) Multiome HiCAR | 204 | |
| chr8:144,372,497–144,372,883 | 71.7 kb | Distal (>10kb) Multiome | 160 | |
| chr8:144,372,993–144,375,084 | 70.5 kb | Distal (>10kb) Multiome HiCAR | 903 | |
| chr8:144,408,968–144,410,189 | 35.0 kb | Distal (>10kb) Multiome | 920 | |
| chr8:144,427,691–144,429,571 | 15.9 kb | Distal (>10kb) Multiome | 1000 | |
| chr8:144,443,691–144,445,047 | 60 bp | At TSS Multiome | 743 | |
| chr8:144,462,568–144,463,422 | 18.5 kb | Distal (>10kb) Multiome | 548 | |
| chr8:144,463,885–144,468,178 | 21.3 kb | Distal (>10kb) Multiome | 910 | |
| chr8:144,471,960–144,474,391 | 28.1 kb | Distal (>10kb) Multiome | 482 | |
| chr8:144,474,517–144,476,543 | 31.4 kb | Distal (>10kb) Multiome | 321 | |
| chr8:144,477,277–144,478,749 | 33.5 kb | Distal (>10kb) Multiome | 679 | |
| chr8:144,478,851–144,479,509 | 34.9 kb | Distal (>10kb) Multiome | 392 | |
| chr8:144,483,979–144,484,843 | 39.9 kb | Distal (>10kb) Multiome | 125 | |
| chr8:144,495,748–144,496,431 | 51.7 kb | Distal (>10kb) Multiome | 131 | |
| chr8:144,500,182–144,501,505 | 56.6 kb | Distal (>10kb) Multiome | 484 | |
| chr8:144,507,995–144,510,268 | 64.4 kb | Distal (>10kb) Multiome | 1067 | |
| chr8:144,517,168–144,518,442 | 73.5 kb | Distal (>10kb) Multiome | 637 | |
| chr8:144,521,935–144,523,450 | 78.1 kb | Distal (>10kb) Multiome | 663 | |
| chr8:144,524,105–144,525,099 | 80.2 kb | Distal (>10kb) Multiome | 264 | |
| chr8:144,526,399–144,529,305 | 82.6 kb | Distal (>10kb) Multiome | 934 | |
| chr8:144,529,455–144,530,630 | 85.7 kb | Distal (>10kb) Multiome HiCAR | 284 | |
| chr8:144,580,290–144,581,657 | 136.3 kb | Distal (>10kb) Multiome | 271 | |
| chr8:144,672,507–144,672,989 | 228.4 kb | Distal (>10kb) Multiome | 150 | |
| chr8:144,684,108–144,686,340 | 240.0 kb | Distal (>10kb) Multiome | 862 |
Genomic view of the TONSL locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.