Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). Troponin I is useful in making a diagnosis of heart failure, and of ischemic heart disease. An elevated level of troponin is also now used as indicator of acute myocardial injury in patients hospitalized with moderate/severe Coronavirus Disease 2019 (COVID-19). Such elevation has also been associated with higher risk of mortality in cardiovascular disease patients hospitalized due to COVID-19. [provided by RefSeq, Aug 2020]
Transcription factors with Perturb-seq knockdown data for TNNI3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TNNI3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TNNI3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:55,062,536–55,063,406 | 93.6 kb | Distal (>10kb) Multiome | 586 | |
| chr19:55,080,038–55,082,744 | 75.2 kb | Distal (>10kb) Multiome | 639 | |
| chr19:55,086,246–55,087,525 | 69.6 kb | Distal (>10kb) Multiome | 258 | |
| chr19:55,091,780–55,093,292 | 64.5 kb | Distal (>10kb) Multiome | 208 | |
| chr19:55,095,398–55,095,977 | 61.0 kb | Distal (>10kb) Multiome | 90 | |
| chr19:55,117,134–55,119,109 | 37.9 kb | Distal (>10kb) Multiome | 881 | |
| chr19:55,146,210–55,148,117 | 9.9 kb | Proximal (<10kb) Multiome | 528 | |
| chr19:55,156,193–55,157,266 | 116 bp | At TSS Multiome | 317 | |
| chr19:55,160,567–55,161,360 | 4.0 kb | Proximal (<10kb) Multiome | 554 | |
| chr19:55,165,952–55,167,256 | 9.9 kb | Proximal (<10kb) Multiome | 719 | |
| chr19:55,173,717–55,174,287 | 17.3 kb | Distal (>10kb) Multiome | 330 | |
| chr19:55,178,876–55,179,477 | 22.3 kb | Distal (>10kb) Multiome | 479 | |
| chr19:55,216,490–55,217,227 | 60.0 kb | Distal (>10kb) Multiome | 1077 | |
| chr19:55,257,751–55,259,635 | 102.4 kb | Distal (>10kb) Multiome | 1054 | |
| chr19:55,279,599–55,280,933 | 123.5 kb | Distal (>10kb) Multiome | 805 | |
| chr19:55,283,835–55,284,716 | 127.2 kb | Distal (>10kb) Multiome | 242 | |
| chr19:55,301,640–55,302,240 | 145.2 kb | Distal (>10kb) Multiome | 448 | |
| chr19:55,338,676–55,340,358 | 182.8 kb | Distal (>10kb) Multiome | 882 | |
| chr19:55,353,720–55,354,487 | 197.3 kb | Distal (>10kb) Multiome | 297 | |
| chr19:55,369,099–55,370,447 | 213.3 kb | Distal (>10kb) Multiome | 383 | |
| chr19:55,383,539–55,384,553 | 227.1 kb | Distal (>10kb) Multiome | 374 | |
| chr19:55,384,697–55,386,560 | 228.9 kb | Distal (>10kb) Multiome | 903 | |
| chr19:55,406,950–55,408,675 | 251.3 kb | Distal (>10kb) Multiome | 965 | |
| chr19:55,452,738–55,453,308 | 296.3 kb | Distal (>10kb) Multiome | 339 |
Genomic view of the TNNI3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.