Predicted to be involved in cardiac conduction; cardiac ventricle development; and regulation of cardiac conduction. Located in several cellular components, including intercalated disc; mitochondrial inner membrane; and nucleolus. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for TMEM65. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TMEM65 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TMEM65, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:124,223,096–124,223,793 | 149.3 kb | Distal (>10kb) Multiome | 60 | |
| chr8:124,235,723–124,236,382 | 136.8 kb | Distal (>10kb) Multiome | 107 | |
| chr8:124,369,228–124,369,416 | 3.3 kb | Proximal (<10kb) | 26 | |
| chr8:124,371,408–124,373,569 | 58 bp | At TSS Multiome | 891 | |
| chr8:124,425,336–124,426,470 | 53.5 kb | Distal (>10kb) Multiome | 332 | |
| chr8:124,473,813–124,475,636 | 102.1 kb | Distal (>10kb) Multiome | 970 | |
| chr8:124,538,847–124,539,474 | 166.4 kb | Distal (>10kb) Multiome | 921 | |
| chr8:124,635,289–124,635,776 | 262.9 kb | Distal (>10kb) Multiome | 230 |
Genomic view of the TMEM65 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.