Transcription factors with Perturb-seq knockdown data for TMEM109-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TMEM109-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TMEM109-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:60,906,047–60,907,006 | 7.0 kb | Proximal (<10kb) | 787 | |
| chr11:60,913,771–60,914,957 | at TSS | At TSS | 710 | |
| chr11:60,915,279–60,915,720 | 1.2 kb | Proximal (<10kb) | 296 | |
| chr11:60,918,351–60,918,949 | 4.3 kb | Proximal (<10kb) | 62 | |
| chr11:60,923,955–60,925,328 | 9.9 kb | Proximal (<10kb) | 668 |
Genomic view of the TMEM109-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.