The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Transcription factors with Perturb-seq knockdown data for TLK1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TLK1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TLK1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:170,873,044–170,873,708 | 287.6 kb | Distal (>10kb) Multiome | 509 | |
| chr2:170,917,144–170,918,588 | 243.1 kb | Distal (>10kb) Multiome | 221 | |
| chr2:170,928,198–170,930,434 | 231.8 kb | Distal (>10kb) Multiome | 1135 | |
| chr2:170,973,304–170,974,525 | 187.3 kb | Distal (>10kb) Multiome HiCAR | 906 | |
| chr2:171,159,743–171,161,450 | 26 bp | At TSS Multiome | 897 | |
| chr2:171,260,495–171,261,286 | 100.0 kb | Distal (>10kb) Multiome | 332 | |
| chr2:171,316,174–171,316,627 | 155.5 kb | Distal (>10kb) Multiome | 85 | |
| chr2:171,433,096–171,434,895 | 273.2 kb | Distal (>10kb) Multiome HiCAR | 1119 |
Genomic view of the TLK1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.