The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. [provided by RefSeq, Oct 2016]
Transcription factors with Perturb-seq knockdown data for TK1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TK1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TK1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:77,915,676–77,916,129 | 271.1 kb | Distal (>10kb) Multiome | 105 | |
| chr17:77,935,380–77,936,363 | 251.2 kb | Distal (>10kb) Multiome | 171 | |
| chr17:77,957,585–77,959,667 | 228.5 kb | Distal (>10kb) Multiome | 1117 | |
| chr17:78,127,361–78,129,338 | 58.3 kb | Distal (>10kb) Multiome | 909 | |
| chr17:78,140,136–78,141,130 | 46.5 kb | Distal (>10kb) Multiome | 594 | |
| chr17:78,168,117–78,169,078 | 18.5 kb | Distal (>10kb) Multiome | 738 | |
| chr17:78,186,463–78,187,739 | 172 bp | At TSS Multiome | 1021 | |
| chr17:78,213,978–78,214,702 | 27.2 kb | Distal (>10kb) Multiome | 902 | |
| chr17:78,231,715–78,232,315 | 45.0 kb | Distal (>10kb) Multiome | 147 | |
| chr17:78,250,689–78,251,651 | 64.0 kb | Distal (>10kb) Multiome | 714 | |
| chr17:78,254,178–78,254,892 | 67.5 kb | Distal (>10kb) Multiome | 860 | |
| chr17:78,270,255–78,273,257 | 85.4 kb | Distal (>10kb) Multiome | 667 | |
| chr17:78,278,089–78,278,902 | 91.5 kb | Distal (>10kb) Multiome | 225 | |
| chr17:78,313,762–78,314,579 | 127.2 kb | Distal (>10kb) Multiome | 636 | |
| chr17:78,341,604–78,342,374 | 155.0 kb | Distal (>10kb) Multiome | 423 | |
| chr17:78,351,461–78,352,240 | 164.7 kb | Distal (>10kb) Multiome | 383 | |
| chr17:78,352,598–78,353,774 | 166.0 kb | Distal (>10kb) Multiome | 687 | |
| chr17:78,356,201–78,356,710 | 169.4 kb | Distal (>10kb) Multiome | 319 | |
| chr17:78,358,869–78,361,525 | 173.2 kb | Distal (>10kb) Multiome | 898 | |
| chr17:78,365,478–78,366,101 | 178.8 kb | Distal (>10kb) Multiome | 305 | |
| chr17:78,378,324–78,379,091 | 191.6 kb | Distal (>10kb) Multiome | 921 |
Genomic view of the TK1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.