This gene encodes a component of the nuclear THO transcription elongation complex, which is part of the larger transcription export (TREX) complex that couples messenger RNA processing and export. In humans, the transcription export complex is recruited to the 5'-end of messenger RNAs in a splicing- and cap-dependent manner. Studies of a related complex in mouse suggest that the metazoan transcription export complex is involved in cell differentiation and development. A pseudogene of this gene has been defined on chromosome 5. [provided by RefSeq, May 2013]
Transcription factors with Perturb-seq knockdown data for THOC3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = THOC3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of THOC3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:175,688,357–175,689,084 | 279.5 kb | Distal (>10kb) Multiome | 66 | |
| chr5:175,729,513–175,730,170 | 238.3 kb | Distal (>10kb) Multiome | 464 | |
| chr5:175,796,457–175,798,050 | 171.2 kb | Distal (>10kb) Multiome | 1084 | |
| chr5:175,871,487–175,872,415 | 96.5 kb | Distal (>10kb) Multiome | 832 | |
| chr5:176,172,154–176,172,641 | 204.3 kb | Distal (>10kb) Multiome | 15 | |
| chr5:176,183,360–176,184,067 | 215.5 kb | Distal (>10kb) Multiome | 165 |
Genomic view of the THOC3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.