Protein kinase B (PKB) is a major downstream target of receptor tyrosine kinases that signal via phosphatidylinositol 3-kinase. Upon cell stimulation, PKB is translocated to the plasma membrane, where it is phosphorylated in the C-terminal regulatory domain. The protein encoded by this gene negatively regulates PKB activity by inhibiting phosphorylation. Transcription of this gene is commonly downregulated in glioblastomas. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for THEM4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = THEM4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of THEM4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:151,611,789–151,613,070 | 297.4 kb | Distal (>10kb) Multiome | 761 | |
| chr1:151,721,370–151,721,843 | 187.9 kb | Distal (>10kb) Multiome | 186 | |
| chr1:151,762,987–151,763,859 | 146.0 kb | Distal (>10kb) Multiome | 848 | |
| chr1:151,790,055–151,791,612 | 118.9 kb | Distal (>10kb) Multiome | 953 | |
| chr1:151,839,238–151,840,386 | 69.7 kb | Distal (>10kb) Multiome | 493 | |
| chr1:151,905,792–151,906,021 | 3.5 kb | Proximal (<10kb) | 111 | |
| chr1:151,909,150–151,910,123 | 59 bp | At TSS Multiome | 983 | |
| chr1:151,936,340–151,937,120 | 27.4 kb | Distal (>10kb) Multiome | 185 | |
| chr1:151,992,346–151,994,801 | 84.5 kb | Distal (>10kb) Multiome | 709 | |
| chr1:152,034,408–152,035,398 | 125.3 kb | Distal (>10kb) Multiome | 590 | |
| chr1:152,036,267–152,037,471 | 127.5 kb | Distal (>10kb) Multiome | 586 | |
| chr1:152,047,642–152,048,726 | 138.6 kb | Distal (>10kb) Multiome | 810 |
Genomic view of the THEM4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.