Transcription factors with Perturb-seq knockdown data for TENM3-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TENM3-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TENM3-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:181,949,492–181,950,812 | 135.0 kb | Distal (>10kb) Multiome | 242 | |
| chr4:182,085,079–182,085,364 | at TSS | At TSS | 85 | |
| chr4:182,091,144–182,091,678 | 6.1 kb | Proximal (<10kb) | 28 | |
| chr4:182,142,242–182,145,566 | 57.7 kb | Distal (>10kb) Multiome | 624 | |
| chr4:182,339,241–182,339,846 | 254.5 kb | Distal (>10kb) Multiome | 163 |
Genomic view of the TENM3-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.