This gene encodes a subunit of a large protein complex known as a vacuolar H+-ATPase (V-ATPase). The protein complex acts as a pump to move protons across the membrane. This movement of protons helps regulate the pH of cells and their surrounding environment. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Alternative splicing results in multiple transcript variants. Mutations in this gene are associated with infantile malignant osteopetrosis. [provided by RefSeq, May 2017]
Transcription factors with Perturb-seq knockdown data for TCIRG1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TCIRG1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TCIRG1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:67,757,971–67,758,804 | 280.7 kb | Distal (>10kb) Multiome | 61 | |
| chr11:67,804,576–67,806,617 | 233.3 kb | Distal (>10kb) Multiome | 372 | |
| chr11:67,955,492–67,956,004 | 83.2 kb | Distal (>10kb) Multiome | 140 | |
| chr11:67,996,583–67,997,678 | 42.0 kb | Distal (>10kb) Multiome | 394 | |
| chr11:68,002,991–68,004,268 | 35.0 kb | Distal (>10kb) Multiome | 634 | |
| chr11:68,030,139–68,031,153 | 8.4 kb | Proximal (<10kb) Multiome | 708 | |
| chr11:68,038,378–68,039,498 | 82 bp | At TSS Multiome | 819 | |
| chr11:68,040,369–68,040,559 | 1.3 kb | Proximal (<10kb) | 412 | |
| chr11:68,120,465–68,122,314 | 82.5 kb | Distal (>10kb) Multiome | 921 | |
| chr11:68,127,759–68,129,017 | 89.4 kb | Distal (>10kb) Multiome | 604 | |
| chr11:68,212,619–68,214,642 | 173.9 kb | Distal (>10kb) Multiome | 967 | |
| chr11:68,271,213–68,272,543 | 233.0 kb | Distal (>10kb) Multiome | 943 | |
| chr11:68,312,404–68,312,863 | 273.6 kb | Distal (>10kb) Multiome | 279 |
Genomic view of the TCIRG1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.