Enables DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in several processes, including negative regulation of transcription by RNA polymerase II; regulation of cell population proliferation; and spermatogenesis. Located in nucleus. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for TCFL5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TCFL5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TCFL5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:62,641,913–62,643,114 | 219.4 kb | Distal (>10kb) Multiome | 874 | |
| chr20:62,791,452–62,791,941 | 70.1 kb | Distal (>10kb) Multiome | 645 | |
| chr20:62,793,003–62,795,717 | 67.4 kb | Distal (>10kb) Multiome | 1051 | |
| chr20:62,796,248–62,797,075 | 65.2 kb | Distal (>10kb) Multiome | 566 | |
| chr20:62,815,479–62,817,462 | 44.8 kb | Distal (>10kb) Multiome | 503 | |
| chr20:62,824,643–62,825,353 | 36.7 kb | Distal (>10kb) Multiome | 339 | |
| chr20:62,860,497–62,862,892 | 183 bp | At TSS Multiome | 996 | |
| chr20:62,900,083–62,900,558 | 38.6 kb | Distal (>10kb) Multiome | 764 | |
| chr20:62,925,950–62,926,856 | 64.8 kb | Distal (>10kb) Multiome | 874 | |
| chr20:62,928,980–62,929,573 | 67.6 kb | Distal (>10kb) Multiome | 289 | |
| chr20:62,937,285–62,938,569 | 76.2 kb | Distal (>10kb) Multiome | 1026 | |
| chr20:63,006,367–63,007,246 | 145.2 kb | Distal (>10kb) Multiome | 189 | |
| chr20:63,071,594–63,072,696 | 210.2 kb | Distal (>10kb) Multiome | 246 |
Genomic view of the TCFL5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.