This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human homolog of mouse Tbx4, which is closely linked to Tbx2 on mouse chromosome 11. Similarly this gene, like TBX2, maps to human chromosome 17. Expression studies in mouse and chicken show that Tbx4 is expressed in developing hindlimb, but not in forelimb buds, suggesting a role for this gene in regulating limb development and specification of limb identity. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for TBX4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TBX4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TBX4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:61,450,785–61,450,946 | 1.5 kb | Proximal (<10kb) | 37 | |
| chr17:61,451,345–61,452,822 | at TSS | At TSS | 253 | |
| chr17:61,454,364–61,455,326 | 2.0 kb | Proximal (<10kb) | 441 | |
| chr17:61,456,171–61,456,729 | 3.8 kb | Proximal (<10kb) | 96 | |
| chr17:61,457,476–61,457,985 | 5.1 kb | Proximal (<10kb) | 74 | |
| chr17:61,461,961–61,462,715 | 9.6 kb | Proximal (<10kb) | 207 |
Genomic view of the TBX4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.