The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. The protein is also an important kinase for antiviral innate immunity response. [provided by RefSeq, Sep 2021]
Transcription factors with Perturb-seq knockdown data for TBK1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TBK1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TBK1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:64,221,528–64,222,890 | 229.9 kb | Distal (>10kb) Multiome | 970 | |
| chr12:64,390,059–64,390,977 | 61.5 kb | Distal (>10kb) Multiome | 268 | |
| chr12:64,403,794–64,405,423 | 47.8 kb | Distal (>10kb) Multiome | 1007 | |
| chr12:64,450,854–64,451,462 | 667 bp | At TSS | 195 | |
| chr12:64,451,663–64,452,624 | 11 bp | At TSS Multiome | 977 | |
| chr12:64,543,521–64,544,182 | 91.6 kb | Distal (>10kb) Multiome | 54 | |
| chr12:64,608,914–64,611,341 | 158.3 kb | Distal (>10kb) Multiome | 922 | |
| chr12:64,681,872–64,683,616 | 230.8 kb | Distal (>10kb) Multiome | 397 |
Genomic view of the TBK1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.