This gene encodes a protein that contains a protein kinase domain, a Rhodanase-like domain and the Tre-2/Bub2/Cdc16 (TBC) domain. The encoded protein is thought to play a role in actin organization, cell growth and cell proliferation by regulating the mammalian target of the rapamycin (mTOR) signaling pathway. This protein may also be involved in the transcriptional regulation of the components of the mTOR complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
Transcription factors with Perturb-seq knockdown data for TBCK. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TBCK upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TBCK, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:106,303,016–106,304,360 | 12.4 kb | Distal (>10kb) Multiome | 141 | |
| chr4:106,310,336–106,310,630 | 5.6 kb | Proximal (<10kb) | 12 | |
| chr4:106,315,490–106,317,043 | 90 bp | At TSS Multiome | 903 | |
| chr4:106,321,972–106,322,424 | 5.7 kb | Proximal (<10kb) | 24 | |
| chr4:106,418,081–106,419,107 | 102.4 kb | Distal (>10kb) Multiome | 127 | |
| chr4:106,501,809–106,502,862 | 186.1 kb | Distal (>10kb) Multiome | 204 | |
| chr4:106,550,844–106,551,984 | 235.2 kb | Distal (>10kb) Multiome | 177 | |
| chr4:106,803,548–106,804,144 | 487.7 kb | Distal (>10kb) Multiome HiCAR | 105 |
Genomic view of the TBCK locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.