TAF3

TATA-box binding protein associated factor 3 | TAF140, TAFII140

The highly conserved RNA polymerase II transcription factor TFIID (see TAF1; MIM 313650) comprises the TATA box-binding protein (TBP; MIM 600075) and a set of TBP-associated factors (TAFs), including TAF3. TAFs contribute to promoter recognition and selectivity and act as antiapoptotic factors (Gangloff et al., 2001 [PubMed 11438666]).[supplied by OMIM, May 2009]

Member of: DE-3 DE-3.1

Biological processes 27 terms

RNA polymerase II general transcription initiation factor activity (GO:0016251)RNA polymerase II preinitiation complex assembly (GO:0051123)chromatin organization (GO:0006325)histone H3K4me3 reader activity (GO:0140002)mRNA transcription by RNA polymerase II (GO:0042789)maintenance of protein location in nucleus (GO:0051457)negative regulation of signal transduction by p53 class mediator (GO:1901797)negative regulation of signal transduction by p53 class mediator (GO:1901797)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription by RNA polymerase II (GO:0000122)nuclear membrane (GO:0031965)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)p53 binding (GO:0002039)p53 binding (GO:0002039)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription initiation by RNA polymerase II (GO:0060261)protein binding (GO:0005515)protein heterodimerization activity (GO:0046982)transcription by RNA polymerase II (GO:0006366)transcription factor TFIID complex (GO:0005669)transcription factor TFIID complex (GO:0005669)transcription regulator inhibitor activity (GO:0140416)transcription regulator inhibitor activity (GO:0140416)

Expression (TPM)

TFs regulating TAF3 0 TFs

Transcription factors with Perturb-seq knockdown data for TAF3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TAF3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to TAF3

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TAF3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr10:7,540,399–7,542,174	277.6 kb	Distal (>10kb) Multiome	420
chr10:7,551,477–7,552,685	266.4 kb	Distal (>10kb) Multiome	160
chr10:7,605,258–7,606,229	212.7 kb	Distal (>10kb) Multiome	80
chr10:7,618,865–7,620,103	198.9 kb	Distal (>10kb) Multiome	246
chr10:7,667,487–7,668,354	150.8 kb	Distal (>10kb) Multiome	159
chr10:7,787,377–7,789,109	30.4 kb	Distal (>10kb) Multiome	843
chr10:7,814,994–7,815,407	3.1 kb	Proximal (<10kb)	5
chr10:7,817,713–7,817,876	626 bp	At TSS	43
chr10:7,818,056–7,819,396	31 bp	At TSS Multiome	897
chr10:8,044,436–8,045,614	226.8 kb	Distal (>10kb) Multiome	246
chr10:8,047,568–8,048,598	229.6 kb	Distal (>10kb) Multiome	190
chr10:8,049,343–8,052,545	231.3 kb	Distal (>10kb) Multiome	796
chr10:8,053,150–8,055,390	235.0 kb	Distal (>10kb) Multiome	590
chr10:8,071,272–8,072,513	253.4 kb	Distal (>10kb) Multiome	160

Genome Browser

Genomic view of the TAF3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.