DNA-binding transcriptional activator proteins increase the rate of transcription by interacting with the transcriptional machinery bound to the basal promoter in conjunction with adaptor proteins, possibly by acetylation and destabilization of nucleosomes. The protein encoded by this gene is a transcriptional activator adaptor and a component of the histone acetyl transferase (HAT) coactivator complex which plays a crucial role in chromatin modulation and cell cycle progression. Along with the other components of the complex, this protein links transcriptional activators bound to specific promoters, to histone acetylation and the transcriptional machinery. The protein is also involved in the stabilization and activation of the p53 tumor suppressor protein that plays a role in the cellular response to DNA damage. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]
Transcription factors with Perturb-seq knockdown data for TADA3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TADA3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TADA3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:9,499,948–9,500,976 | 292.1 kb | Distal (>10kb) Multiome | 320 | |
| chr3:9,552,048–9,552,879 | 240.3 kb | Distal (>10kb) Multiome | 372 | |
| chr3:9,553,391–9,554,161 | 238.9 kb | Distal (>10kb) Multiome | 128 | |
| chr3:9,649,068–9,649,746 | 143.4 kb | Distal (>10kb) Multiome | 793 | |
| chr3:9,703,572–9,705,701 | 87.6 kb | Distal (>10kb) Multiome | 664 | |
| chr3:9,727,013–9,727,518 | 65.5 kb | Distal (>10kb) Multiome | 264 | |
| chr3:9,730,724–9,732,470 | 61.4 kb | Distal (>10kb) Multiome | 934 | |
| chr3:9,749,252–9,750,525 | 42.8 kb | Distal (>10kb) Multiome | 873 | |
| chr3:9,769,503–9,770,124 | 22.8 kb | Distal (>10kb) Multiome | 504 | |
| chr3:9,792,176–9,793,536 | 75 bp | At TSS Multiome | 915 | |
| chr3:9,809,609–9,810,744 | 17.4 kb | Distal (>10kb) Multiome | 437 | |
| chr3:9,843,116–9,844,397 | 51.2 kb | Distal (>10kb) Multiome | 787 | |
| chr3:9,862,465–9,863,231 | 70.1 kb | Distal (>10kb) Multiome | 581 | |
| chr3:9,890,247–9,891,058 | 97.9 kb | Distal (>10kb) Multiome | 969 | |
| chr3:9,902,504–9,903,128 | 110.0 kb | Distal (>10kb) Multiome | 396 | |
| chr3:9,914,983–9,916,160 | 122.5 kb | Distal (>10kb) Multiome | 668 | |
| chr3:9,916,644–9,917,412 | 124.3 kb | Distal (>10kb) Multiome | 519 | |
| chr3:9,932,470–9,934,327 | 140.1 kb | Distal (>10kb) Multiome | 1035 | |
| chr3:9,946,152–9,947,739 | 153.9 kb | Distal (>10kb) Multiome | 898 | |
| chr3:9,951,898–9,952,503 | 159.6 kb | Distal (>10kb) Multiome | 484 | |
| chr3:9,986,547–9,987,409 | 194.2 kb | Distal (>10kb) Multiome | 907 | |
| chr3:10,008,678–10,009,458 | 216.4 kb | Distal (>10kb) Multiome | 240 | |
| chr3:10,010,580–10,011,700 | 218.5 kb | Distal (>10kb) Multiome | 540 | |
| chr3:10,025,904–10,026,762 | 233.6 kb | Distal (>10kb) Multiome | 871 |
Genomic view of the TADA3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.