Transcription factors with Perturb-seq knockdown data for SYBU-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SYBU-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SYBU-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:109,639,698–109,639,888 | 4.2 kb | Proximal (<10kb) | 126 | |
| chr8:109,642,607–109,642,836 | 1.3 kb | Proximal (<10kb) | 144 | |
| chr8:109,643,972–109,645,811 | at TSS | At TSS | 815 | |
| chr8:109,648,635–109,648,861 | 4.5 kb | Proximal (<10kb) | 22 | |
| chr8:109,650,984–109,651,222 | 6.9 kb | Proximal (<10kb) | 15 | |
| chr8:109,652,521–109,652,711 | 8.4 kb | Proximal (<10kb) | 81 |
Genomic view of the SYBU-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.