The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known. [provided by RefSeq, Sep 2009]
Transcription factors with Perturb-seq knockdown data for STRADA. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = STRADA upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of STRADA, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:63,445,554–63,447,581 | 295.6 kb | Distal (>10kb) Multiome | 896 | |
| chr17:63,476,359–63,478,095 | 264.5 kb | Distal (>10kb) Multiome | 520 | |
| chr17:63,478,783–63,479,370 | 262.7 kb | Distal (>10kb) Multiome | 419 | |
| chr17:63,522,589–63,523,803 | 218.6 kb | Distal (>10kb) Multiome | 705 | |
| chr17:63,549,660–63,551,443 | 191.5 kb | Distal (>10kb) Multiome | 1037 | |
| chr17:63,600,715–63,601,423 | 140.9 kb | Distal (>10kb) Multiome | 1122 | |
| chr17:63,621,512–63,623,359 | 119.8 kb | Distal (>10kb) Multiome | 956 | |
| chr17:63,698,705–63,701,428 | 41.6 kb | Distal (>10kb) Multiome | 663 | |
| chr17:63,741,119–63,742,935 | 60 bp | At TSS Multiome | 1057 | |
| chr17:63,773,179–63,774,727 | 32.0 kb | Distal (>10kb) Multiome | 1169 | |
| chr17:63,826,794–63,828,024 | 85.6 kb | Distal (>10kb) Multiome | 1008 | |
| chr17:63,841,547–63,843,442 | 101.2 kb | Distal (>10kb) Multiome | 1203 | |
| chr17:63,848,836–63,849,566 | 107.4 kb | Distal (>10kb) Multiome | 658 | |
| chr17:63,960,119–63,962,604 | 220.1 kb | Distal (>10kb) Multiome | 445 | |
| chr17:63,998,051–63,998,826 | 256.6 kb | Distal (>10kb) Multiome | 408 |
Genomic view of the STRADA locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.