Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. [provided by RefSeq, Apr 2020]
Transcription factors with Perturb-seq knockdown data for STAU1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = STAU1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of STAU1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:48,921,125–48,922,305 | 266.6 kb | Distal (>10kb) Multiome | 1077 | |
| chr20:49,045,736–49,047,213 | 141.8 kb | Distal (>10kb) Multiome | 1069 | |
| chr20:49,187,732–49,189,017 | 96 bp | At TSS Multiome | 893 | |
| chr20:49,218,926–49,220,026 | 31.0 kb | Distal (>10kb) Multiome | 836 | |
| chr20:49,277,517–49,279,612 | 90.0 kb | Distal (>10kb) Multiome | 1199 | |
| chr20:49,289,765–49,291,410 | 102.0 kb | Distal (>10kb) Multiome | 436 | |
| chr20:49,482,537–49,483,070 | 294.6 kb | Distal (>10kb) Multiome | 732 | |
| chr20:49,483,315–49,484,620 | 296.0 kb | Distal (>10kb) Multiome | 684 |
Genomic view of the STAU1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.