This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
Transcription factors with Perturb-seq knockdown data for SQSTM1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SQSTM1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SQSTM1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:179,530,398–179,530,934 | 290.2 kb | Distal (>10kb) Multiome | 322 | |
| chr5:179,549,624–179,551,424 | 270.1 kb | Distal (>10kb) Multiome | 799 | |
| chr5:179,559,012–179,560,188 | 261.3 kb | Distal (>10kb) Multiome | 624 | |
| chr5:179,622,406–179,625,135 | 198.2 kb | Distal (>10kb) Multiome | 1217 | |
| chr5:179,678,118–179,679,585 | 142.3 kb | Distal (>10kb) Multiome | 1007 | |
| chr5:179,698,113–179,699,498 | 122.1 kb | Distal (>10kb) Multiome | 1062 | |
| chr5:179,732,076–179,733,697 | 88.2 kb | Distal (>10kb) Multiome | 820 | |
| chr5:179,795,059–179,796,988 | 24.7 kb | Distal (>10kb) Multiome | 778 | |
| chr5:179,805,642–179,807,610 | 14.0 kb | Distal (>10kb) Multiome | 747 | |
| chr5:179,816,597–179,817,385 | 3.5 kb | Proximal (<10kb) | 160 | |
| chr5:179,818,809–179,822,095 | 49 bp | At TSS Multiome | 1243 | |
| chr5:179,858,283–179,859,465 | 38.0 kb | Distal (>10kb) Multiome | 923 | |
| chr5:179,907,372–179,908,268 | 87.0 kb | Distal (>10kb) Multiome | 528 | |
| chr5:180,007,647–180,008,169 | 186.9 kb | Distal (>10kb) Multiome | 251 | |
| chr5:180,059,605–180,060,144 | 238.9 kb | Distal (>10kb) Multiome | 18 | |
| chr5:180,069,680–180,070,372 | 249.0 kb | Distal (>10kb) Multiome | 240 | |
| chr5:180,071,104–180,072,473 | 251.1 kb | Distal (>10kb) Multiome | 1084 | |
| chr5:180,090,117–180,091,054 | 269.6 kb | Distal (>10kb) Multiome | 575 |
Genomic view of the SQSTM1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.