Serine palmitoyltransferase (SPT; EC 2.3.1.50) catalyzes the first committed and rate-limiting step in sphingolipid biosynthesis. SSSPTA is a small SPT subunit that stimulates SPT activity and confers acyl-CoA preference to the SPT catalytic heterodimer of SPTLC1 (MIM 605712) and either SPTLC2 (MIM 605713) or SPTLC3 (MIM 611120) (Han et al., 2009 [PubMed 19416851]).[supplied by OMIM, Nov 2010]
Transcription factors with Perturb-seq knockdown data for SPTSSA. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SPTSSA upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SPTSSA, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:34,331,736–34,332,458 | 130.1 kb | Distal (>10kb) Multiome | 698 | |
| chr14:34,460,930–34,461,135 | 1.1 kb | Proximal (<10kb) | 214 | |
| chr14:34,461,634–34,463,144 | 110 bp | At TSS Multiome | 931 | |
| chr14:34,629,461–34,630,514 | 167.9 kb | Distal (>10kb) Multiome | 907 | |
| chr14:34,693,166–34,693,989 | 231.1 kb | Distal (>10kb) Multiome | 463 | |
| chr14:34,713,982–34,715,044 | 252.4 kb | Distal (>10kb) Multiome | 919 |
Genomic view of the SPTSSA locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.