SMAD6

SMAD6

SMAD family member 6 | HsT17432, MADH6, MADH7

The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014]

Member of: DE-3 Developmental clusters: GC6

Biological processes 78 terms

BMP signaling pathway (GO:0030509)BMP signaling pathway (GO:0030509)I-SMAD binding (GO:0070411)I-SMAD binding (GO:0070411)I-SMAD binding (GO:0070411)R-SMAD binding (GO:0070412)SMAD protein signal transduction (GO:0060395)activin receptor binding (GO:0070697)anatomical structure morphogenesis (GO:0009653)aortic valve morphogenesis (GO:0003180)cell differentiation (GO:0030154)cell-substrate adhesion (GO:0031589)cellular response to growth factor stimulus (GO:0071363)chromatin (GO:0000785)chromatin binding (GO:0003682)ciliary basal body (GO:0036064)co-SMAD binding (GO:0070410)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytosol (GO:0005829)fat cell differentiation (GO:0045444)heteromeric SMAD protein complex (GO:0071144)identical protein binding (GO:0042802)immune response (GO:0006955)mitral valve morphogenesis (GO:0003183)mitral valve morphogenesis (GO:0003183)negative regulation of BMP signaling pathway (GO:0030514)negative regulation of BMP signaling pathway (GO:0030514)negative regulation of BMP signaling pathway (GO:0030514)negative regulation of MyD88-dependent toll-like receptor signaling pathway (GO:0034125)negative regulation of SMAD protein signal transduction (GO:0060392)negative regulation of SMAD protein signal transduction (GO:0060392)negative regulation of SMAD protein signal transduction (GO:0060392)negative regulation of activin receptor signaling pathway (GO:0032926)negative regulation of activin receptor signaling pathway (GO:0032926)negative regulation of apoptotic process (GO:0043066)negative regulation of cell differentiation (GO:0045596)negative regulation of cell population proliferation (GO:0008285)negative regulation of ossification (GO:0030279)negative regulation of ossification (GO:0030279)negative regulation of osteoblast differentiation (GO:0045668)negative regulation of osteoblast differentiation (GO:0045668)negative regulation of transforming growth factor beta receptor signaling pathway (GO:0030512)negative regulation of transforming growth factor beta receptor signaling pathway (GO:0030512)negative regulation of transforming growth factor beta receptor signaling pathway (GO:0030512)nuclear body (GO:0016604)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)outflow tract septum morphogenesis (GO:0003148)outflow tract septum morphogenesis (GO:0003148)positive regulation of miRNA transcription (GO:1902895)proteasome-mediated ubiquitin-dependent protein catabolic process (GO:0043161)protein binding (GO:0005515)protein sequestering activity (GO:0140311)protein-containing complex (GO:0032991)pulmonary valve morphogenesis (GO:0003184)pulmonary valve morphogenesis (GO:0003184)regulation of DNA-templated transcription (GO:0006355)regulation of transcription by RNA polymerase II (GO:0006357)response to estrogen (GO:0043627)response to laminar fluid shear stress (GO:0034616)response to lipopolysaccharide (GO:0032496)transcription cis-regulatory region binding (GO:0000976)transcription regulator complex (GO:0005667)transcription regulator inhibitor activity (GO:0140416)transcription regulator inhibitor activity (GO:0140416)transcription regulator inhibitor activity (GO:0140416)transcription regulator inhibitor activity (GO:0140416)transforming growth factor beta receptor superfamily signaling pathway (GO:0141091)type I activin receptor binding (GO:0070698)type I transforming growth factor beta receptor binding (GO:0034713)type I transforming growth factor beta receptor binding (GO:0034713)ubiquitin protein ligase binding (GO:0031625)ubiquitin-like ligase-substrate adaptor activity (GO:1990756)ureteric bud development (GO:0001657)zygotic specification of dorsal/ventral axis (GO:0007352)

Expression (TPM)

TFs regulating SMAD6 0 TFs

Transcription factors with Perturb-seq knockdown data for SMAD6. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SMAD6 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to SMAD6

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SMAD6, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility	Element	Dist. to TSS	Link type	TFs
	chr15:66,472,216–66,473,164	229.7 kb	Distal (>10kb) Multiome	208
	chr15:66,497,104–66,498,247	204.5 kb	Distal (>10kb) Multiome	1044
	chr15:66,504,543–66,505,655	197.1 kb	Distal (>10kb) Multiome	940
	chr15:66,567,056–66,567,492	135.0 kb	Distal (>10kb) Multiome	294
	chr15:66,592,463–66,593,143	109.5 kb	Distal (>10kb) Multiome HiCAR	154
	chr15:66,621,672–66,622,446	80.0 kb	Distal (>10kb) Multiome	271
	chr15:66,630,856–66,631,340	71.1 kb	Distal (>10kb) Multiome	284
	chr15:66,670,796–66,671,740	31.0 kb	Distal (>10kb) Multiome	241
	chr15:66,673,331–66,673,903	28.5 kb	Distal (>10kb) Multiome	192
	chr15:66,679,362–66,680,264	22.4 kb	Distal (>10kb) Multiome	541
	chr15:66,691,568–66,692,527	10.1 kb	Distal (>10kb) Multiome	33
	chr15:66,698,421–66,699,404	3.1 kb	Proximal (<10kb) Multiome	321
	chr15:66,700,619–66,702,650	1.1 kb	Proximal (<10kb) Multiome	844
	chr15:66,707,348–66,707,765	5.1 kb	Proximal (<10kb)	182
	chr15:66,773,918–66,774,633	72.2 kb	Distal (>10kb) Multiome	524
	chr15:66,842,072–66,842,664	140.4 kb	Distal (>10kb) Multiome	519
	chr15:66,842,765–66,843,830	141.1 kb	Distal (>10kb) Multiome	413
	chr15:66,923,439–66,924,007	221.5 kb	Distal (>10kb) Multiome	191
	chr15:66,927,757–66,928,460	226.0 kb	Distal (>10kb) Multiome	77

Genome Browser

Genomic view of the SMAD6 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.