SIX4
SIX homeobox 4 | AREC3

This gene encodes a member of the homeobox family, subfamily SIX. The drosophila homolog is a nuclear homeoprotein required for eye development. Studies in mouse show that this gene product functions as a transcription factor, and may have a role in the differentiation or maturation of neuronal cells. [provided by RefSeq, May 2010]

Developmental clusters: GC4
Biological processes 68 terms
DNA binding (GO:0003677)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)anatomical structure morphogenesis (GO:0009653)chromatin (GO:0000785)cytoplasm (GO:0005737)cytoplasm (GO:0005737)fungiform papilla morphogenesis (GO:0061197)fungiform papilla morphogenesis (GO:0061197)generation of neurons (GO:0048699)generation of neurons (GO:0048699)male gonad development (GO:0008584)male gonad development (GO:0008584)male sex determination (GO:0030238)male sex determination (GO:0030238)male sex differentiation (GO:0046661)male sex differentiation (GO:0046661)metanephric mesenchyme development (GO:0072075)metanephric mesenchyme development (GO:0072075)myotome development (GO:0061055)myotome development (GO:0061055)negative regulation of DNA-templated transcription (GO:0045892)negative regulation of DNA-templated transcription (GO:0045892)negative regulation of apoptotic process (GO:0043066)negative regulation of apoptotic process (GO:0043066)negative regulation of neuron apoptotic process (GO:0043524)negative regulation of neuron apoptotic process (GO:0043524)negative regulation of satellite cell differentiation (GO:1902725)negative regulation of satellite cell differentiation (GO:1902725)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)olfactory placode formation (GO:0030910)olfactory placode formation (GO:0030910)pharyngeal system development (GO:0060037)pharyngeal system development (GO:0060037)positive regulation of DNA-templated transcription (GO:0045893)positive regulation of branching involved in ureteric bud morphogenesis (GO:0090190)positive regulation of branching involved in ureteric bud morphogenesis (GO:0090190)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of ureteric bud formation (GO:0072107)positive regulation of ureteric bud formation (GO:0072107)protein localization to nucleus (GO:0034504)protein localization to nucleus (GO:0034504)regulation of DNA-templated transcription (GO:0006355)regulation of branch elongation involved in ureteric bud branching (GO:0072095)regulation of branch elongation involved in ureteric bud branching (GO:0072095)regulation of epithelial cell proliferation (GO:0050678)regulation of epithelial cell proliferation (GO:0050678)regulation of transcription by RNA polymerase II (GO:0006357)sarcomere organization (GO:0045214)sarcomere organization (GO:0045214)sequence-specific DNA binding (GO:0043565)sequence-specific double-stranded DNA binding (GO:1990837)skeletal muscle fiber differentiation (GO:0098528)skeletal muscle fiber differentiation (GO:0098528)skeletal muscle tissue development (GO:0007519)skeletal muscle tissue development (GO:0007519)tongue development (GO:0043586)tongue development (GO:0043586)transcription regulator complex (GO:0005667)trigeminal ganglion development (GO:0061551)trigeminal ganglion development (GO:0061551)
Expression (TPM)
SIX4 — as a Regulated Gene

TFs regulating SIX4 0 TFs

Transcription factors with Perturb-seq knockdown data for SIX4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SIX4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to SIX4

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SIX4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr14:60,485,178–60,486,252 238.4 kb Distal (>10kb) Multiome 378
chr14:60,508,535–60,510,247 215.3 kb Distal (>10kb) Multiome 245
chr14:60,514,579–60,516,786 209.4 kb Distal (>10kb) Multiome 517
chr14:60,637,200–60,638,337 86.5 kb Distal (>10kb) Multiome 201
chr14:60,641,851–60,643,218 81.9 kb Distal (>10kb) Multiome 222
chr14:60,648,454–60,651,189 73.5 kb Distal (>10kb) Multiome 790
chr14:60,655,802–60,657,953 67.1 kb Distal (>10kb) Multiome 631
chr14:60,720,299–60,724,768 17 bp At TSS Multiome 933
chr14:60,726,393–60,726,699 2.0 kb Proximal (<10kb) 2
chr14:60,726,940–60,727,551 2.6 kb Proximal (<10kb) 16
chr14:60,980,625–60,981,938 256.8 kb Distal (>10kb) Multiome HiCAR 866

Genome Browser

Genomic view of the SIX4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr14:60,475,178 – 60,991,938
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq