This gene encodes an enzyme that catalyzes the production of monoselenophosphate (MSP) from selenide and ATP. MSP is the selenium donor required for synthesis of selenocysteine (Sec), which is co-translationally incorporated into selenoproteins at in-frame UGA codons that normally signal translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is itself a selenoprotein containing a Sec residue at its active site, suggesting the existence of an autoregulatory mechanism. It is preferentially expressed in tissues implicated in the synthesis of selenoproteins and in sites of blood cell development. A pseudogene for this locus has been identified on chromosome 5. [provided by RefSeq, May 2017]
Transcription factors with Perturb-seq knockdown data for SEPHS2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SEPHS2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SEPHS2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:30,182,759–30,183,865 | 262.4 kb | Distal (>10kb) Multiome | 547 | |
| chr16:30,334,526–30,335,777 | 110.5 kb | Distal (>10kb) Multiome | 486 | |
| chr16:30,354,854–30,355,978 | 90.5 kb | Distal (>10kb) Multiome | 1070 | |
| chr16:30,369,220–30,371,611 | 75.1 kb | Distal (>10kb) Multiome | 818 | |
| chr16:30,377,495–30,379,532 | 67.5 kb | Distal (>10kb) Multiome | 1025 | |
| chr16:30,394,422–30,396,098 | 50.7 kb | Distal (>10kb) Multiome | 941 | |
| chr16:30,400,026–30,401,048 | 45.3 kb | Distal (>10kb) Multiome | 839 | |
| chr16:30,406,974–30,408,429 | 38.4 kb | Distal (>10kb) Multiome | 908 | |
| chr16:30,417,414–30,418,576 | 27.8 kb | Distal (>10kb) Multiome | 543 | |
| chr16:30,429,571–30,430,773 | 15.7 kb | Distal (>10kb) Multiome | 1060 | |
| chr16:30,437,340–30,437,978 | 7.9 kb | Proximal (<10kb) | 329 | |
| chr16:30,445,340–30,446,062 | 51 bp | At TSS Multiome | 898 | |
| chr16:30,526,068–30,527,896 | 81.5 kb | Distal (>10kb) Multiome | 1004 | |
| chr16:30,534,370–30,535,460 | 89.0 kb | Distal (>10kb) Multiome HiCAR | 957 | |
| chr16:30,557,913–30,558,847 | 112.6 kb | Distal (>10kb) Multiome | 1025 | |
| chr16:30,571,001–30,572,505 | 126.2 kb | Distal (>10kb) Multiome | 950 | |
| chr16:30,585,243–30,586,223 | 139.9 kb | Distal (>10kb) Multiome | 588 | |
| chr16:30,609,586–30,610,748 | 164.6 kb | Distal (>10kb) Multiome HiCAR | 1030 | |
| chr16:30,634,070–30,634,986 | 188.5 kb | Distal (>10kb) Multiome HiCAR | 907 | |
| chr16:30,650,029–30,651,831 | 204.8 kb | Distal (>10kb) Multiome | 992 | |
| chr16:30,657,638–30,659,894 | 212.4 kb | Distal (>10kb) Multiome | 1053 | |
| chr16:30,677,814–30,678,622 | 232.3 kb | Distal (>10kb) Multiome | 177 | |
| chr16:30,697,755–30,699,614 | 253.2 kb | Distal (>10kb) Multiome | 1153 |
Genomic view of the SEPHS2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.