Stearoyl-CoA desaturase (SCD; EC 1.14.99.5) is an integral membrane protein of the endoplasmic reticulum that catalyzes the formation of monounsaturated fatty acids from saturated fatty acids. SCD may be a key regulator of energy metabolism with a role in obesity and dislipidemia. Four SCD isoforms, Scd1 through Scd4, have been identified in mouse. In contrast, only 2 SCD isoforms, SCD1 (MIM 604031) and SCD5, have been identified in human. SCD1 shares about 85% amino acid identity with all 4 mouse SCD isoforms, as well as with rat Scd1 and Scd2. In contrast, SCD5 shares limited homology with the rodent SCDs and appears to be unique to primates (Wang et al., 2005 [PubMed 15907797]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for SCD5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SCD5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SCD5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:82,561,307–82,562,355 | 236.9 kb | Distal (>10kb) Multiome | 327 | |
| chr4:82,667,793–82,668,466 | 130.6 kb | Distal (>10kb) Multiome | 123 | |
| chr4:82,716,050–82,716,644 | 82.4 kb | Distal (>10kb) Multiome | 173 | |
| chr4:82,739,190–82,739,774 | 59.3 kb | Distal (>10kb) Multiome | 317 | |
| chr4:82,797,244–82,798,074 | 1.3 kb | Proximal (<10kb) Multiome | 243 | |
| chr4:82,798,169–82,799,103 | 35 bp | At TSS Multiome | 368 | |
| chr4:82,799,242–82,799,535 | 446 bp | At TSS | 85 | |
| chr4:82,890,719–82,891,825 | 92.5 kb | Distal (>10kb) Multiome | 1005 | |
| chr4:82,899,856–82,901,464 | 101.9 kb | Distal (>10kb) Multiome | 977 | |
| chr4:83,010,137–83,011,179 | 212.0 kb | Distal (>10kb) Multiome | 601 | |
| chr4:83,012,161–83,013,775 | 214.3 kb | Distal (>10kb) Multiome | 913 | |
| chr4:83,034,341–83,035,668 | 236.2 kb | Distal (>10kb) Multiome | 993 |
Genomic view of the SCD5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.