Sterile alpha motifs (SAMs) in proteins such as SAMD4A are part of an RNA-binding domain that functions as a posttranscriptional regulator by binding to an RNA sequence motif known as the Smaug recognition element, which was named after the Drosophila Smaug protein (Baez and Boccaccio, 2005 [PubMed 16221671]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for SAMD4A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SAMD4A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SAMD4A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:54,361,880–54,362,724 | 203.8 kb | Distal (>10kb) Multiome | 146 | |
| chr14:54,366,777–54,367,506 | 199.0 kb | Distal (>10kb) Multiome | 393 | |
| chr14:54,396,464–54,397,605 | 169.3 kb | Distal (>10kb) Multiome | 808 | |
| chr14:54,440,764–54,441,825 | 124.8 kb | Distal (>10kb) Multiome | 860 | |
| chr14:54,488,387–54,489,472 | 77.2 kb | Distal (>10kb) Multiome | 922 | |
| chr14:54,509,366–54,510,519 | 56.3 kb | Distal (>10kb) Multiome | 801 | |
| chr14:54,564,898–54,567,596 | 106 bp | At TSS Multiome | 963 | |
| chr14:54,567,716–54,568,327 | 1.9 kb | Proximal (<10kb) Multiome | 296 | |
| chr14:54,572,407–54,572,592 | 6.3 kb | Proximal (<10kb) | 98 | |
| chr14:54,773,202–54,773,766 | 207.3 kb | Distal (>10kb) Multiome HiCAR | 548 | |
| chr14:54,805,654–54,806,307 | 239.8 kb | Distal (>10kb) Multiome HiCAR | 376 |
Genomic view of the SAMD4A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.