Transcription factors with Perturb-seq knockdown data for RWDD2A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RWDD2A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RWDD2A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:82,246,942–82,248,798 | 945.5 kb | Distal (>10kb) Multiome HiCAR | 957 | |
| chr6:83,065,409–83,066,246 | 127.5 kb | Distal (>10kb) Multiome | 863 | |
| chr6:83,067,273–83,069,447 | 125.4 kb | Distal (>10kb) Multiome | 1006 | |
| chr6:83,188,335–83,188,952 | 4.4 kb | Proximal (<10kb) | 70 | |
| chr6:83,190,736–83,190,920 | 2.4 kb | Proximal (<10kb) | 26 | |
| chr6:83,192,782–83,194,476 | 33 bp | At TSS Multiome | 948 | |
| chr6:83,200,602–83,200,767 | 7.2 kb | Proximal (<10kb) | 3 | |
| chr6:83,430,232–83,432,291 | 237.9 kb | Distal (>10kb) Multiome | 784 | |
| chr6:83,511,480–83,513,126 | 319.0 kb | Distal (>10kb) Multiome HiCAR | 450 |
Genomic view of the RWDD2A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.