This gene encodes a RPIP8, UNC-14, and NESCA domain-containing protein that is required for maintenance of neuronal polarity. In addition, it has been implicated in mediation of gastric cancer cell migration and invasion via interaction with P21-activated kinase-1, which promotes its expression. The encoded protein localizes to F-actin-enriched invadopodia to induce formation of protrusions, thereby facilitating cell migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
Transcription factors with Perturb-seq knockdown data for RUFY3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RUFY3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RUFY3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:70,474,006–70,475,095 | 230.2 kb | Distal (>10kb) Multiome | 187 | |
| chr4:70,687,549–70,689,032 | 16.5 kb | Distal (>10kb) Multiome | 1053 | |
| chr4:70,703,846–70,706,278 | 98 bp | At TSS Multiome | 934 | |
| chr4:70,713,057–70,713,685 | 8.3 kb | Proximal (<10kb) | 183 | |
| chr4:70,836,603–70,837,949 | 132.4 kb | Distal (>10kb) Multiome | 136 | |
| chr4:70,838,760–70,840,084 | 135.1 kb | Distal (>10kb) Multiome | 828 | |
| chr4:70,901,672–70,903,467 | 197.5 kb | Distal (>10kb) Multiome HiCAR | 915 | |
| chr4:70,992,414–70,994,232 | 288.8 kb | Distal (>10kb) Multiome | 926 |
Genomic view of the RUFY3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.