This gene encodes a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]
Transcription factors with Perturb-seq knockdown data for RSPO4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RSPO4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RSPO4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:727,954–728,888 | 273.8 kb | Distal (>10kb) Multiome | 621 | |
| chr20:775,602–776,151 | 226.3 kb | Distal (>10kb) Multiome | 126 | |
| chr20:833,367–834,334 | 168.6 kb | Distal (>10kb) Multiome | 839 | |
| chr20:841,821–842,567 | 159.9 kb | Distal (>10kb) Multiome | 248 | |
| chr20:844,437–845,353 | 157.7 kb | Distal (>10kb) Multiome | 299 | |
| chr20:884,981–885,530 | 117.1 kb | Distal (>10kb) Multiome | 183 | |
| chr20:1,001,978–1,002,815 | 93 bp | At TSS Multiome | 124 | |
| chr20:1,004,172–1,004,579 | 1.9 kb | Proximal (<10kb) | 22 | |
| chr20:1,005,564–1,005,927 | 3.3 kb | Proximal (<10kb) | 124 | |
| chr20:1,118,327–1,119,071 | 116.3 kb | Distal (>10kb) Multiome | 1066 | |
| chr20:1,184,818–1,186,197 | 183.4 kb | Distal (>10kb) Multiome | 557 | |
| chr20:1,225,365–1,226,806 | 223.5 kb | Distal (>10kb) Multiome | 616 | |
| chr20:1,266,029–1,266,958 | 264.2 kb | Distal (>10kb) Multiome | 499 |
Genomic view of the RSPO4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.