This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and ATF1. The encoded protein can also phosphorylate histone H3 to regulate certain inflammatory genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
Transcription factors with Perturb-seq knockdown data for RPS6KA4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RPS6KA4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RPS6KA4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:64,165,638–64,166,698 | 192.9 kb | Distal (>10kb) Multiome | 811 | |
| chr11:64,184,927–64,186,817 | 173.2 kb | Distal (>10kb) Multiome | 1091 | |
| chr11:64,225,548–64,226,862 | 133.0 kb | Distal (>10kb) Multiome | 776 | |
| chr11:64,230,074–64,231,130 | 128.7 kb | Distal (>10kb) Multiome | 715 | |
| chr11:64,233,950–64,235,296 | 124.7 kb | Distal (>10kb) Multiome | 590 | |
| chr11:64,240,398–64,242,687 | 117.9 kb | Distal (>10kb) Multiome | 982 | |
| chr11:64,246,274–64,248,065 | 112.0 kb | Distal (>10kb) Multiome | 802 | |
| chr11:64,250,699–64,251,842 | 107.7 kb | Distal (>10kb) Multiome | 498 | |
| chr11:64,268,509–64,270,920 | 89.1 kb | Distal (>10kb) Multiome | 1093 | |
| chr11:64,271,352–64,271,994 | 87.6 kb | Distal (>10kb) Multiome | 595 | |
| chr11:64,284,095–64,286,666 | 73.4 kb | Distal (>10kb) Multiome | 807 | |
| chr11:64,300,129–64,301,268 | 58.1 kb | Distal (>10kb) Multiome | 591 | |
| chr11:64,304,032–64,307,072 | 52.8 kb | Distal (>10kb) Multiome | 994 | |
| chr11:64,316,954–64,319,418 | 40.1 kb | Distal (>10kb) Multiome | 1039 | |
| chr11:64,340,711–64,341,211 | 18.2 kb | Distal (>10kb) Multiome | 212 | |
| chr11:64,342,083–64,343,227 | 16.5 kb | Distal (>10kb) Multiome | 543 | |
| chr11:64,355,132–64,355,542 | 3.6 kb | Proximal (<10kb) | 605 | |
| chr11:64,358,792–64,359,751 | 9 bp | At TSS Multiome | 577 | |
| chr11:64,448,870–64,449,995 | 89.8 kb | Distal (>10kb) Multiome | 490 | |
| chr11:64,630,081–64,630,643 | 271.1 kb | Distal (>10kb) Multiome | 226 | |
| chr11:64,637,888–64,638,531 | 279.1 kb | Distal (>10kb) Multiome | 168 | |
| chr11:64,656,654–64,657,126 | 297.8 kb | Distal (>10kb) Multiome HiCAR | 80 |
Genomic view of the RPS6KA4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.