Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L27e family of ribosomal proteins and a component of the 60S subunit. A splice site mutation in this gene has been identified in a Diamond-Blackfan anemia (DBA) patient. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Mar 2017]
Transcription factors with Perturb-seq knockdown data for RPL27. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RPL27 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RPL27, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:42,744,352–42,745,375 | 253.5 kb | Distal (>10kb) Multiome | 966 | |
| chr17:42,761,003–42,761,468 | 237.4 kb | Distal (>10kb) Multiome | 335 | |
| chr17:42,780,029–42,780,899 | 217.9 kb | Distal (>10kb) Multiome | 712 | |
| chr17:42,798,408–42,798,877 | 199.7 kb | Distal (>10kb) Multiome | 866 | |
| chr17:42,823,968–42,824,503 | 174.1 kb | Distal (>10kb) Multiome | 829 | |
| chr17:42,832,889–42,833,841 | 165.1 kb | Distal (>10kb) Multiome | 980 | |
| chr17:42,964,237–42,964,843 | 33.9 kb | Distal (>10kb) Multiome | 655 | |
| chr17:42,979,966–42,981,044 | 18.0 kb | Distal (>10kb) Multiome | 843 | |
| chr17:42,997,598–42,998,917 | 110 bp | At TSS Multiome | 1112 | |
| chr17:43,021,026–43,022,568 | 24.0 kb | Distal (>10kb) Multiome | 973 | |
| chr17:43,024,255–43,025,752 | 26.6 kb | Distal (>10kb) Multiome | 811 | |
| chr17:43,124,981–43,125,702 | 127.1 kb | Distal (>10kb) Multiome | 837 | |
| chr17:43,169,744–43,171,576 | 172.7 kb | Distal (>10kb) Multiome | 1018 | |
| chr17:43,211,494–43,212,392 | 213.5 kb | Distal (>10kb) Multiome | 586 |
Genomic view of the RPL27 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.