The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1.[provided by RefSeq, Jul 2010]
Transcription factors with Perturb-seq knockdown data for RNF4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RNF4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RNF4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:2,241,509–2,242,666 | 226.9 kb | Distal (>10kb) Multiome | 783 | |
| chr4:2,261,141–2,263,069 | 206.8 kb | Distal (>10kb) Multiome | 806 | |
| chr4:2,401,939–2,402,479 | 66.8 kb | Distal (>10kb) Multiome | 317 | |
| chr4:2,413,008–2,413,630 | 55.7 kb | Distal (>10kb) Multiome | 564 | |
| chr4:2,417,912–2,419,213 | 50.3 kb | Distal (>10kb) Multiome | 628 | |
| chr4:2,462,245–2,463,183 | 6.4 kb | Proximal (<10kb) Multiome | 643 | |
| chr4:2,468,399–2,469,652 | 103 bp | At TSS Multiome | 858 | |
| chr4:2,469,747–2,469,937 | 644 bp | At TSS | 442 | |
| chr4:2,535,307–2,537,785 | 67.3 kb | Distal (>10kb) Multiome | 860 | |
| chr4:2,755,816–2,757,021 | 287.2 kb | Distal (>10kb) Multiome | 760 | |
| chr4:2,763,947–2,764,427 | 295.2 kb | Distal (>10kb) Multiome | 311 |
Genomic view of the RNF4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.