Transcription factors with Perturb-seq knockdown data for RNF217-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RNF217-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RNF217-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:124,955,086–124,955,732 | 7.4 kb | Proximal (<10kb) | 96 | |
| chr6:124,961,736–124,963,798 | 1.0 kb | Proximal (<10kb) Multiome | 660 | |
| chr6:125,099,255–125,100,053 | 136.5 kb | Distal (>10kb) Multiome | 540 | |
| chr6:125,153,568–125,155,310 | 191.8 kb | Distal (>10kb) Multiome HiCAR | 927 | |
| chr6:125,952,889–125,953,903 | 990.3 kb | Distal (>10kb) Multiome HiCAR | 343 | |
| chr6:125,956,540–125,957,527 | 993.7 kb | Distal (>10kb) Multiome HiCAR | 1085 |
Genomic view of the RNF217-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.