Transcription factors with Perturb-seq knockdown data for RNA5SP75. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RNA5SP75 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RNA5SP75, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:204,707,179–204,707,469 | at TSS | At TSS | 80 | |
| chr1:204,709,678–204,711,587 | 2.2 kb | Proximal (<10kb) | 521 | |
| chr1:204,712,071–204,712,719 | 4.6 kb | Proximal (<10kb) | 173 | |
| chr1:204,714,466–204,715,865 | 7.0 kb | Proximal (<10kb) | 356 |
Genomic view of the RNA5SP75 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.