This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
Transcription factors with Perturb-seq knockdown data for RHOA. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RHOA upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RHOA, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:49,120,575–49,121,282 | 291.1 kb | Distal (>10kb) Multiome | 765 | |
| chr3:49,132,603–49,133,965 | 278.3 kb | Distal (>10kb) Multiome | 652 | |
| chr3:49,166,057–49,166,586 | 245.7 kb | Distal (>10kb) Multiome | 622 | |
| chr3:49,170,896–49,171,950 | 240.6 kb | Distal (>10kb) Multiome | 781 | |
| chr3:49,276,912–49,277,398 | 134.9 kb | Distal (>10kb) Multiome | 291 | |
| chr3:49,339,607–49,340,853 | 71.8 kb | Distal (>10kb) Multiome | 1044 | |
| chr3:49,357,868–49,359,083 | 53.6 kb | Distal (>10kb) Multiome | 908 | |
| chr3:49,411,409–49,412,844 | 164 bp | At TSS Multiome | 899 | |
| chr3:49,422,179–49,422,905 | 10.5 kb | Distal (>10kb) Multiome | 453 | |
| chr3:49,428,857–49,429,869 | 17.5 kb | Distal (>10kb) Multiome | 712 | |
| chr3:49,461,484–49,462,134 | 49.7 kb | Distal (>10kb) Multiome | 207 | |
| chr3:49,468,926–49,470,930 | 58.2 kb | Distal (>10kb) Multiome | 919 | |
| chr3:49,539,369–49,540,584 | 128.0 kb | Distal (>10kb) Multiome | 628 | |
| chr3:49,553,618–49,555,244 | 142.5 kb | Distal (>10kb) Multiome | 710 |
Genomic view of the RHOA locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.