Transcription factors with Perturb-seq knockdown data for RFPL1S. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RFPL1S upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RFPL1S, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:29,205,602–29,206,473 | 272.1 kb | Distal (>10kb) Multiome | 378 | |
| chr22:29,267,574–29,268,917 | 210.0 kb | Distal (>10kb) Multiome | 969 | |
| chr22:29,304,664–29,307,808 | 171.3 kb | Distal (>10kb) Multiome | 839 | |
| chr22:29,313,065–29,313,784 | 164.8 kb | Distal (>10kb) Multiome | 274 | |
| chr22:29,315,074–29,316,064 | 162.6 kb | Distal (>10kb) Multiome | 242 | |
| chr22:29,388,161–29,388,971 | 89.6 kb | Distal (>10kb) Multiome | 740 | |
| chr22:29,431,916–29,432,715 | 45.9 kb | Distal (>10kb) Multiome | 14 | |
| chr22:29,470,132–29,470,844 | 7.7 kb | Proximal (<10kb) Multiome | 437 | |
| chr22:29,480,003–29,481,437 | 2.9 kb | Proximal (<10kb) Multiome | 384 | |
| chr22:29,553,345–29,553,988 | 75.6 kb | Distal (>10kb) Multiome | 837 | |
| chr22:29,580,084–29,581,520 | 102.9 kb | Distal (>10kb) Multiome | 856 | |
| chr22:29,603,006–29,604,162 | 125.3 kb | Distal (>10kb) Multiome | 803 | |
| chr22:29,766,399–29,767,654 | 288.9 kb | Distal (>10kb) Multiome | 837 |
Genomic view of the RFPL1S locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.