The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for RFC4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RFC4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RFC4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:186,566,830–186,567,930 | 239.1 kb | Distal (>10kb) Multiome | 850 | |
| chr3:186,570,047–186,571,165 | 235.9 kb | Distal (>10kb) Multiome HiCAR | 735 | |
| chr3:186,653,868–186,654,549 | 152.3 kb | Distal (>10kb) Multiome | 163 | |
| chr3:186,772,225–186,772,685 | 33.9 kb | Distal (>10kb) Multiome | 147 | |
| chr3:186,772,788–186,773,308 | 33.5 kb | Distal (>10kb) Multiome | 241 | |
| chr3:186,775,941–186,776,679 | 30.2 kb | Distal (>10kb) Multiome | 161 | |
| chr3:186,782,866–186,784,510 | 22.3 kb | Distal (>10kb) Multiome | 1121 | |
| chr3:186,806,142–186,807,252 | 90 bp | At TSS Multiome | 877 | |
| chr3:186,824,344–186,826,061 | 19.1 kb | Distal (>10kb) Multiome | 328 | |
| chr3:186,827,252–186,828,058 | 21.1 kb | Distal (>10kb) Multiome | 539 | |
| chr3:186,929,799–186,932,446 | 123.9 kb | Distal (>10kb) Multiome | 861 | |
| chr3:186,998,752–186,999,772 | 192.8 kb | Distal (>10kb) Multiome | 215 | |
| chr3:187,025,680–187,026,649 | 219.6 kb | Distal (>10kb) Multiome | 205 |
Genomic view of the RFC4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.