This gene encodes a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival. Disruption of this gene is associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene of this gene has been defined on chromosome 2. [provided by RefSeq, Jul 2013]
Transcription factors with Perturb-seq knockdown data for RFC2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RFC2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RFC2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:74,026,698–74,027,309 | 227.4 kb | Distal (>10kb) Multiome | 294 | |
| chr7:74,067,853–74,068,319 | 186.4 kb | Distal (>10kb) Multiome | 359 | |
| chr7:74,082,928–74,085,020 | 170.7 kb | Distal (>10kb) Multiome | 602 | |
| chr7:74,094,257–74,095,257 | 159.5 kb | Distal (>10kb) Multiome | 409 | |
| chr7:74,173,712–74,175,040 | 80.1 kb | Distal (>10kb) Multiome | 927 | |
| chr7:74,206,458–74,207,053 | 47.6 kb | Distal (>10kb) Multiome | 436 | |
| chr7:74,253,979–74,254,920 | 79 bp | At TSS Multiome | 943 | |
| chr7:74,269,798–74,270,278 | 15.6 kb | Distal (>10kb) Multiome | 527 | |
| chr7:74,282,776–74,283,619 | 28.6 kb | Distal (>10kb) Multiome HiCAR | 157 | |
| chr7:74,284,675–74,285,215 | 30.5 kb | Distal (>10kb) Multiome HiCAR | 204 | |
| chr7:74,288,851–74,289,933 | 35.0 kb | Distal (>10kb) Multiome HiCAR | 529 | |
| chr7:74,371,455–74,372,279 | 117.5 kb | Distal (>10kb) Multiome HiCAR | 234 | |
| chr7:74,453,330–74,454,656 | 199.4 kb | Distal (>10kb) Multiome | 596 |
Genomic view of the RFC2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.