The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for RBL1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RBL1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RBL1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:36,821,902–36,822,404 | 273.9 kb | Distal (>10kb) Multiome | 177 | |
| chr20:36,835,484–36,836,113 | 260.2 kb | Distal (>10kb) Multiome | 446 | |
| chr20:36,846,853–36,847,425 | 248.9 kb | Distal (>10kb) Multiome | 228 | |
| chr20:36,863,126–36,864,425 | 232.1 kb | Distal (>10kb) Multiome | 833 | |
| chr20:36,886,953–36,887,714 | 208.7 kb | Distal (>10kb) Multiome | 586 | |
| chr20:36,951,062–36,952,347 | 144.2 kb | Distal (>10kb) Multiome | 859 | |
| chr20:37,095,487–37,096,500 | 146 bp | At TSS Multiome | 1181 | |
| chr20:37,178,598–37,179,693 | 83.1 kb | Distal (>10kb) Multiome | 977 | |
| chr20:37,289,329–37,289,991 | 193.7 kb | Distal (>10kb) Multiome | 1018 | |
| chr20:37,319,185–37,319,833 | 223.5 kb | Distal (>10kb) Multiome | 87 | |
| chr20:37,345,682–37,346,478 | 250.0 kb | Distal (>10kb) Multiome | 557 |
Genomic view of the RBL1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.