The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Dec 2008]
Transcription factors with Perturb-seq knockdown data for RAD54L. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RAD54L upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RAD54L, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:46,131,887–46,133,691 | 114.8 kb | Distal (>10kb) Multiome | 1126 | |
| chr1:46,198,117–46,198,832 | 49.2 kb | Distal (>10kb) Multiome | 646 | |
| chr1:46,203,021–46,203,710 | 44.3 kb | Distal (>10kb) Multiome | 394 | |
| chr1:46,246,904–46,248,623 | 196 bp | At TSS Multiome | 923 | |
| chr1:46,254,338–46,255,513 | 7.0 kb | Proximal (<10kb) Multiome | 39 | |
| chr1:46,301,645–46,303,965 | 55.8 kb | Distal (>10kb) Multiome | 1035 | |
| chr1:46,340,061–46,341,413 | 93.0 kb | Distal (>10kb) Multiome | 967 | |
| chr1:46,394,021–46,394,849 | 146.7 kb | Distal (>10kb) Multiome | 795 | |
| chr1:46,401,942–46,402,392 | 154.5 kb | Distal (>10kb) Multiome | 511 | |
| chr1:46,406,082–46,407,120 | 158.8 kb | Distal (>10kb) Multiome | 299 | |
| chr1:46,442,171–46,443,351 | 195.0 kb | Distal (>10kb) Multiome | 649 | |
| chr1:46,448,113–46,448,679 | 200.8 kb | Distal (>10kb) Multiome | 143 | |
| chr1:46,466,221–46,467,348 | 219.0 kb | Distal (>10kb) Multiome | 398 | |
| chr1:46,489,045–46,490,312 | 242.0 kb | Distal (>10kb) Multiome | 381 | |
| chr1:46,516,974–46,517,563 | 269.5 kb | Distal (>10kb) Multiome | 328 | |
| chr1:46,525,135–46,525,776 | 277.7 kb | Distal (>10kb) Multiome | 169 | |
| chr1:46,526,636–46,527,311 | 279.3 kb | Distal (>10kb) Multiome | 225 | |
| chr1:46,532,918–46,533,689 | 285.5 kb | Distal (>10kb) Multiome | 254 |
Genomic view of the RAD54L locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.