The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for RAB8A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RAB8A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RAB8A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:15,331,471–15,332,992 | 779.4 kb | Distal (>10kb) Multiome HiCAR | 641 | |
| chr19:16,075,837–16,077,586 | 35.5 kb | Distal (>10kb) Multiome | 735 | |
| chr19:16,110,632–16,112,318 | 240 bp | At TSS Multiome | 866 | |
| chr19:16,184,971–16,186,029 | 73.5 kb | Distal (>10kb) Multiome | 658 | |
| chr19:16,197,472–16,198,501 | 86.0 kb | Distal (>10kb) Multiome | 806 | |
| chr19:16,247,463–16,247,928 | 135.9 kb | Distal (>10kb) Multiome | 370 | |
| chr19:16,324,466–16,326,401 | 214.2 kb | Distal (>10kb) Multiome | 477 |
Genomic view of the RAB8A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.