The protein encoded by this gene catalyzes the formation of tRNA pseudouridine from tRNA uridine at position 39 in the anticodon stem and loop of transfer RNAs. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]
Transcription factors with Perturb-seq knockdown data for PUS3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PUS3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PUS3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:125,625,323–125,626,856 | 277.1 kb | Distal (>10kb) Multiome | 1108 | |
| chr11:125,886,970–125,888,478 | 15.4 kb | Distal (>10kb) Multiome | 823 | |
| chr11:125,902,922–125,903,413 | 60 bp | At TSS Multiome | 495 | |
| chr11:125,904,107–125,904,760 | 1.2 kb | Proximal (<10kb) Multiome | 235 | |
| chr11:125,938,819–125,939,449 | 35.8 kb | Distal (>10kb) Multiome | 161 | |
| chr11:125,941,709–125,942,323 | 38.9 kb | Distal (>10kb) Multiome | 270 | |
| chr11:126,061,736–126,063,712 | 159.9 kb | Distal (>10kb) Multiome | 802 | |
| chr11:126,082,403–126,083,132 | 179.5 kb | Distal (>10kb) Multiome | 134 | |
| chr11:126,104,267–126,104,892 | 201.4 kb | Distal (>10kb) Multiome | 192 | |
| chr11:126,159,698–126,160,582 | 256.9 kb | Distal (>10kb) Multiome | 281 |
Genomic view of the PUS3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.