The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PSMD8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PSMD8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PSMD8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:38,074,116–38,074,898 | 300.2 kb | Distal (>10kb) Multiome | 299 | |
| chr19:38,183,265–38,183,970 | 191.1 kb | Distal (>10kb) Multiome | 156 | |
| chr19:38,223,967–38,224,571 | 150.5 kb | Distal (>10kb) Multiome | 677 | |
| chr19:38,229,570–38,230,021 | 145.0 kb | Distal (>10kb) Multiome | 354 | |
| chr19:38,255,918–38,256,778 | 118.2 kb | Distal (>10kb) Multiome | 371 | |
| chr19:38,263,873–38,265,423 | 110.3 kb | Distal (>10kb) Multiome | 590 | |
| chr19:38,304,071–38,305,583 | 69.5 kb | Distal (>10kb) Multiome | 531 | |
| chr19:38,315,514–38,316,642 | 58.7 kb | Distal (>10kb) Multiome | 747 | |
| chr19:38,319,279–38,320,342 | 54.9 kb | Distal (>10kb) Multiome | 359 | |
| chr19:38,335,358–38,337,514 | 39.0 kb | Distal (>10kb) Multiome | 898 | |
| chr19:38,361,454–38,362,809 | 12.7 kb | Distal (>10kb) Multiome | 604 | |
| chr19:38,374,176–38,375,269 | 130 bp | At TSS Multiome | 1041 | |
| chr19:38,385,485–38,386,937 | 11.2 kb | Distal (>10kb) Multiome | 156 | |
| chr19:38,387,065–38,389,471 | 13.4 kb | Distal (>10kb) Multiome | 591 | |
| chr19:38,394,369–38,395,035 | 19.9 kb | Distal (>10kb) Multiome | 191 | |
| chr19:38,402,504–38,403,738 | 28.3 kb | Distal (>10kb) Multiome | 940 | |
| chr19:38,417,766–38,418,568 | 43.3 kb | Distal (>10kb) Multiome | 407 | |
| chr19:38,434,539–38,435,128 | 60.1 kb | Distal (>10kb) Multiome | 238 | |
| chr19:38,452,620–38,453,510 | 78.4 kb | Distal (>10kb) Multiome | 355 | |
| chr19:38,506,710–38,507,745 | 132.5 kb | Distal (>10kb) Multiome | 369 | |
| chr19:38,514,956–38,515,568 | 140.5 kb | Distal (>10kb) Multiome | 404 | |
| chr19:38,565,090–38,565,701 | 190.6 kb | Distal (>10kb) Multiome | 164 | |
| chr19:38,595,857–38,597,322 | 221.6 kb | Distal (>10kb) Multiome | 478 | |
| chr19:38,617,752–38,619,569 | 244.2 kb | Distal (>10kb) Multiome | 835 | |
| chr19:38,647,137–38,648,653 | 272.8 kb | Distal (>10kb) Multiome | 856 | |
| chr19:38,666,103–38,666,668 | 291.5 kb | Distal (>10kb) Multiome | 317 |
Genomic view of the PSMD8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.