The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
Transcription factors with Perturb-seq knockdown data for PSMC4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PSMC4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PSMC4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:39,793,033–39,793,501 | 177.9 kb | Distal (>10kb) Multiome | 418 | |
| chr19:39,824,152–39,825,056 | 146.5 kb | Distal (>10kb) Multiome | 531 | |
| chr19:39,833,207–39,834,766 | 137.3 kb | Distal (>10kb) Multiome | 779 | |
| chr19:39,845,668–39,847,301 | 124.8 kb | Distal (>10kb) Multiome | 854 | |
| chr19:39,970,370–39,971,611 | 161 bp | At TSS Multiome | 930 | |
| chr19:39,996,681–39,997,502 | 25.9 kb | Distal (>10kb) Multiome | 898 | |
| chr19:40,055,898–40,056,369 | 85.0 kb | Distal (>10kb) Multiome | 746 | |
| chr19:40,090,491–40,091,099 | 119.7 kb | Distal (>10kb) Multiome | 831 | |
| chr19:40,190,883–40,192,408 | 220.7 kb | Distal (>10kb) Multiome | 764 | |
| chr19:40,217,060–40,217,642 | 246.1 kb | Distal (>10kb) Multiome | 366 |
Genomic view of the PSMC4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.