The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]
Transcription factors with Perturb-seq knockdown data for PSMB5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PSMB5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PSMB5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:22,766,419–22,767,375 | 268.2 kb | Distal (>10kb) Multiome | 988 | |
| chr14:22,821,826–22,823,530 | 212.0 kb | Distal (>10kb) Multiome | 649 | |
| chr14:22,829,704–22,830,328 | 205.0 kb | Distal (>10kb) Multiome | 871 | |
| chr14:22,836,107–22,837,017 | 198.4 kb | Distal (>10kb) Multiome | 320 | |
| chr14:22,852,845–22,853,709 | 181.6 kb | Distal (>10kb) Multiome | 788 | |
| chr14:22,870,967–22,873,202 | 163.3 kb | Distal (>10kb) Multiome | 1167 | |
| chr14:22,882,594–22,883,886 | 151.7 kb | Distal (>10kb) Multiome | 289 | |
| chr14:22,886,371–22,887,824 | 147.7 kb | Distal (>10kb) Multiome | 639 | |
| chr14:22,918,527–22,919,975 | 115.6 kb | Distal (>10kb) Multiome | 641 | |
| chr14:22,928,961–22,930,849 | 105.4 kb | Distal (>10kb) Multiome | 1108 | |
| chr14:22,956,530–22,957,512 | 77.8 kb | Distal (>10kb) Multiome | 983 | |
| chr14:22,981,215–22,983,249 | 52.2 kb | Distal (>10kb) Multiome HiCAR | 935 | |
| chr14:23,006,674–23,007,583 | 27.7 kb | Distal (>10kb) Multiome | 1097 | |
| chr14:23,020,642–23,021,291 | 13.9 kb | Distal (>10kb) Multiome | 148 | |
| chr14:23,101,326–23,101,861 | 66.7 kb | Distal (>10kb) Multiome | 83 | |
| chr14:23,154,159–23,154,639 | 119.5 kb | Distal (>10kb) Multiome | 392 | |
| chr14:23,161,040–23,161,530 | 126.5 kb | Distal (>10kb) Multiome | 397 | |
| chr14:23,182,495–23,182,942 | 147.9 kb | Distal (>10kb) Multiome | 139 | |
| chr14:23,237,732–23,238,192 | 203.0 kb | Distal (>10kb) Multiome | 536 | |
| chr14:23,285,641–23,286,830 | 251.4 kb | Distal (>10kb) Multiome | 962 | |
| chr14:23,300,944–23,303,002 | 266.8 kb | Distal (>10kb) Multiome | 1096 | |
| chr14:23,306,324–23,307,321 | 271.9 kb | Distal (>10kb) Multiome | 871 | |
| chr14:23,320,558–23,322,196 | 286.3 kb | Distal (>10kb) Multiome | 1090 |
Genomic view of the PSMB5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.