This gene encodes a protein with a proline-rich domain. This gene is located in a region of chromosome 22 reported to contain a tumor suppressor gene that may be involved in breast and colorectal tumorigenesis. The protein is a component of the mammalian target of rapamycin complex 2 (mTORC2), and it regulates platelet-derived growth factor (PDGF) receptor beta expression and PDGF signaling to Akt and S6K1. Alternative splicing and the use of alternative promoters results in transcripts encoding different isoforms. Read-through transcripts from this gene into the downstream Rho GTPase activating protein 8 (ARHGAP8) gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene. [provided by RefSeq, Nov 2010]
Transcription factors with Perturb-seq knockdown data for PRR5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRR5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRR5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:43,861,938–43,862,881 | 806.2 kb | Distal (>10kb) Multiome HiCAR | 263 | |
| chr22:43,923,130–43,924,772 | 744.9 kb | Distal (>10kb) Multiome HiCAR | 741 | |
| chr22:43,925,117–43,926,046 | 743.1 kb | Distal (>10kb) Multiome HiCAR | 441 | |
| chr22:43,955,027–43,955,843 | 713.2 kb | Distal (>10kb) Multiome HiCAR | 809 | |
| chr22:44,023,900–44,024,505 | 644.5 kb | Distal (>10kb) Multiome HiCAR | 355 | |
| chr22:44,046,421–44,047,108 | 621.8 kb | Distal (>10kb) Multiome HiCAR | 200 | |
| chr22:44,497,886–44,498,569 | 170.4 kb | Distal (>10kb) Multiome | 437 | |
| chr22:44,661,031–44,661,516 | 7.5 kb | Proximal (<10kb) Multiome | 28 | |
| chr22:44,668,163–44,669,181 | 140 bp | At TSS Multiome | 265 | |
| chr22:44,684,755–44,685,227 | 16.2 kb | Distal (>10kb) Multiome | 562 | |
| chr22:44,697,476–44,697,709 | 4.5 kb | Proximal (<10kb) | 320 | |
| chr22:44,701,628–44,703,097 | 33.4 kb | Distal (>10kb) Multiome | 588 | |
| chr22:44,752,293–44,752,996 | 83.9 kb | Distal (>10kb) Multiome | 233 |
Genomic view of the PRR5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.