PRPF6
pre-mRNA processing factor 6 | ANT-1, Prp6, RP60, SNRNP102, TOM, U5-102K, bB152O15.1, hPrp6, C20orf14

The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing. [provided by RefSeq, Jul 2008]

Member of: DE-1 DE-1.5
Biological processes 39 terms
RNA binding (GO:0003723)RNA binding (GO:0003723)RNA localization (GO:0006403)RNA processing (GO:0006396)RNA splicing (GO:0008380)RNA splicing, via transesterification reactions (GO:0000375)U2-type precatalytic spliceosome (GO:0071005)U4/U6 x U5 tri-snRNP complex (GO:0046540)U4/U6 x U5 tri-snRNP complex (GO:0046540)U5 snRNP (GO:0005682)catalytic step 2 spliceosome (GO:0071013)catalytic step 2 spliceosome (GO:0071013)identical protein binding (GO:0042802)mRNA splicing, via spliceosome (GO:0000398)mRNA splicing, via spliceosome (GO:0000398)mRNA splicing, via spliceosome (GO:0000398)mRNA splicing, via spliceosome (GO:0000398)mRNA splicing, via spliceosome (GO:0000398)membrane (GO:0016020)nuclear speck (GO:0016607)nuclear speck (GO:0016607)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)positive regulation of transcription by RNA polymerase II (GO:0045944)protein binding (GO:0005515)protein-macromolecule adaptor activity (GO:0030674)ribonucleoprotein complex binding (GO:0043021)spliceosomal complex (GO:0005681)spliceosomal complex assembly (GO:0000245)spliceosomal snRNP assembly (GO:0000387)spliceosomal tri-snRNP complex assembly (GO:0000244)spliceosomal tri-snRNP complex assembly (GO:0000244)spliceosomal tri-snRNP complex assembly (GO:0000244)spliceosomal tri-snRNP complex assembly (GO:0000244)spliceosomal tri-snRNP complex assembly (GO:0000244)spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) (GO:0000388)
Expression (TPM)
PRPF6 — as a Regulated Gene

TFs regulating PRPF6 0 TFs

Transcription factors with Perturb-seq knockdown data for PRPF6. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRPF6 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to PRPF6

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRPF6, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr20:63,696,254–63,697,070 284.6 kb Distal (>10kb) Multiome 458
chr20:63,707,489–63,708,488 273.2 kb Distal (>10kb) Multiome 1008
chr20:63,737,573–63,741,470 241.5 kb Distal (>10kb) Multiome 1098
chr20:63,775,216–63,775,997 205.7 kb Distal (>10kb) Multiome 257
chr20:63,830,341–63,832,721 149.8 kb Distal (>10kb) Multiome 628
chr20:63,846,273–63,846,790 134.7 kb Distal (>10kb) Multiome 487
chr20:63,864,234–63,865,991 116.0 kb Distal (>10kb) Multiome 1158
chr20:63,894,341–63,895,764 86.1 kb Distal (>10kb) Multiome 791
chr20:63,938,191–63,939,815 42.4 kb Distal (>10kb) Multiome 287
chr20:63,939,918–63,942,008 39.5 kb Distal (>10kb) Multiome 496
chr20:63,955,375–63,956,875 24.8 kb Distal (>10kb) Multiome 1107
chr20:63,956,976–63,958,127 23.5 kb Distal (>10kb) Multiome 709
chr20:63,969,149–63,970,667 11.2 kb Distal (>10kb) Multiome 764
chr20:63,973,660–63,974,150 7.2 kb Proximal (<10kb) Multiome 767
chr20:63,978,681–63,979,797 1.3 kb Proximal (<10kb) 828
chr20:63,979,914–63,980,251 879 bp At TSS 468
chr20:63,980,541–63,981,488 17 bp At TSS Multiome 986
chr20:64,030,226–64,031,221 49.6 kb Distal (>10kb) Multiome 159
chr20:64,038,338–64,039,469 58.0 kb Distal (>10kb) Multiome 628
chr20:64,047,989–64,049,987 68.3 kb Distal (>10kb) Multiome 541
chr20:64,053,902–64,054,682 73.1 kb Distal (>10kb) Multiome 609
chr20:64,057,481–64,058,099 76.8 kb Distal (>10kb) Multiome 659
chr20:64,062,561–64,063,881 82.0 kb Distal (>10kb) Multiome 991
chr20:64,079,311–64,080,472 98.8 kb Distal (>10kb) Multiome 688
chr20:64,083,392–64,084,408 102.6 kb Distal (>10kb) Multiome 591
chr20:64,101,897–64,102,924 121.2 kb Distal (>10kb) Multiome 457
chr20:64,160,199–64,160,889 179.4 kb Distal (>10kb) Multiome 55
chr20:64,255,171–64,256,183 274.5 kb Distal (>10kb) Multiome 861

Genome Browser

Genomic view of the PRPF6 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr20:63,686,254 – 64,266,183
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq